Interactive Tool

Peptide Reconstitution Calculator

Enter how much peptide is in the vial, how much bacteriostatic water you’re adding, and your target research dose. You’ll get the exact volume to draw in U-100 insulin-syringe units, the resulting concentration, and how many doses the vial holds.

How the math works

Reconstitution is just a concentration problem. When you dissolve a lyophilized peptide in bacteriostatic water, the concentration is the peptide amount divided by the water volume:

Concentration

Concentration (mg/mL) = peptide in vial (mg) ÷ water added (mL). Multiply by 1000 to get mcg/mL.

To find the volume for a target dose, divide the dose by the concentration. On a U-100 insulin syringe, every 0.01 mL equals 1 unit, so multiplying the volume in mL by 100 gives the units to draw:

Volume & units

Volume (mL) = dose (mcg) ÷ concentration (mcg/mL)  •  Units (U-100) = volume (mL) × 100

Worked example

A 10 mg vial reconstituted with 2 mL of water gives 5 mg/mL, or 5000 mcg/mL. For a 250 mcg target dose: 250 ÷ 5000 = 0.05 mL = 5 units. The vial holds 10,000 mcg ÷ 250 = 40 doses.

Read the full reconstitution guide →

Research-use note. This calculator is provided for educational and laboratory measurement purposes only. The compounds referenced on this site are investigational and not approved for human or veterinary use. Nothing here is medical advice or a dosing recommendation.